Integrating design and function

Learn how our intuitively designed cartridge architecture incorporates the versatile Xceler8™ Technology to fully automate nucleic acid extraction and markedly simplify molecular research and testing.

Cartridge architecture
Load sample
Prepare reagents
Mix samples with reagents to effectively lyse cells
Fill channel to selectively bind & gently crosslink nucleic acids
Carefully wash away impurities using controlled fluidics
Subtly release purified nucleic acid from surface
Elute purified nucleic acid sample
Retrieve sealed cartridge & high-quality nucleic acid

Breakthrough technology for beyond simple automation

Representing a departure from conventional, labor-intensive and time-consuming sample preparation methods, the disruptive Xceler8 Technology allows users to extract and purify nucleic acids from a variety of human, bacterial and viral samples at the push of a button.

Slide 1

Overview

Unlike typical commercial spin-column or magnetic-bead technologies, the automated Xceler8 Technology uses a novel four-step chemistry-based approach to purify nucleic acids.

Slide 2

Cell lysis

Once the sample has been added and the X8 Cartridge inserted into the Instrument, the sample is mixed with a lysis buffer and loaded into the channel. Incubation with the buffer lyses the cells to liberate the nucleic acids.

Slide 3

Selective binding

A reversible cross-linker then selectively binds and gently clusters the nucleic acids on to the solid surface of the main channel, forming a loose 3-D network.

Slide 4

Wash away impurities

Impurities and cellular debris are then washed away and sealed in a waste reservoir within the cartridge, leaving behind purified nucleic acids, thereby simplifying clean-up and minimizing contamination.

Slide 5

Elute nucleic acid

The purified nucleic acids are then released from the surface of the channel and eluted into the collection tube, now ready for a wide range of research and clinical applications.

Automated HMW DNA extraction with the X8 HMW DNA Cartridge Kit

Slide 1

HMW DNA extraction performance using X8. Pulse-Field Gel Electrophoresis (PFGE) analysis of HMW DNA extracted from three different sample types using the automated X8 HMW DNA Cartridge Kit. Lanes1-5, replicates of 2×106 PBMC. Lanes 6-10, replicates of frozen Rat Liver tissue (20 mg). Lanes 11-13, replicates of 1×106 HeLa cells (UHMW protocol) and Lanes 14-16, replicates of 1×106 HeLa cells (HMW protocol). M= CHEF DNA Size Marker, 0.2-2.2 Mb.

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High RNA Yield with the X8 Cellular RNA Cartridge Kit

Slide 2

Cellular RNA extraction performance using X8. RNA was isolated in parallel from 6 samples of HeLa cell pellets containing 1 X 106 HeLa cells each. The quality of the total RNA was determined with the Agilent 2100 Bioanalyzer and Nanodrop. Elution volume was 80-90 μl.

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High DNA yield with the X8 Genomic DNA Cartridge Kit

Slide 3

Genomic DNA extraction performance using X8 Vs. competitor manual kits. Agarose gel analysis of purified DNA from cell culture, gram- & gram+ bacterial cultures; Quantified DNA yield & RT-PCR data from various gram+ bacterial cultures; Ct values (inversely proportional to yield).

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High DNA Yield with the X8 Tissue DNA Cartridge

Slide 4

Tissue DNA extraction performance using X8. Analysis of DNA isolated from frozen Rat Tail, Liver, Heart and Lung tissue respectively, using a manual kit from Company Q and replicates of automated X8 Tissue DNA Cartridge Kit.

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Fit seamlessly into your existing workflow

Application Notes

Genomic DNA from Cell Culture Learn More
Genomic DNA from Bacteria Learn More

Downstream Applications

Next Generation Sequencing Learn More
Long Read Sequencing Coming soon